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. 2023 Mar 15;14(3):197. doi: 10.1038/s41419-023-05722-2

Fig. 3. Genotoxic stress increases TSPYL2 protein stability in female but not in male cancer cells.

Fig. 3

A Western blot analysis of TSPYL2 levels in U2OS and B MG-63 cells treated with etoposide for 18 h and added with cycloheximide (CHX) for 3, 5, 7 h. C Western blot analysis of TSPYL2 immunoprecipitates from U2OS and D MG-63 cells transfected with HA-Ubiquitin plasmid, in untreated condition and after 8 h of etoposide treatment. E Western blot analysis of TSPYL2 immunoprecipitates from U2OS cells transfected with FLAG-TSPYL2 and MYC-MDM2, in untreated condition and after 24 h of etoposide treatment. F Western blot analysis of TSPYL2 induction at 24 h after etoposide treatment in MDM2 depleted U2OS and G MG-63 cells. H Western blot analysis of TSPYL2 immunoprecipitates from MDM2 depleted U2OS and I MG-63 cells transfected with HA-Ubiquitin plasmid, in untreated condition and after 8 h of etoposide treatment.