Figure 1.

RNAi targeting pteridine synthesis pathway in Oncopeltus. Photos of nymphs and adults, as indicated. (A) Offspring of females injected with a gfp dsRNA control exhibit wild type phenotypes at (i) first, (ii) second, (iii) third, and (iv) fourth instars. (B) Offspring of females injected with Of-ok1 dsRNA were indistinguishable from controls. pRNAi knockdown of (C) Of-xdh1, (D) Of-xdh2, or (E) Of-xdh1 and Of-xdh2. Knockdown of Of-xdh1 caused a lightening of body color from red (A) to yellow-orange, particular evident in second through fourth instars (Cii–iv; Eii–iv). (F,G) Nymphal RNAi. The eye color of last instar nymphs and adults are similar in controls (Fi,ii) and after nRNAi^ok1 (Gi,ii).