Figure EV1. Brd4 promotes Th2 cell differentiation.

1. Flow cytometry analysis of mouse Th2 cells treated with JQ1 as indicated. Mouse Th2 cells were differentiated from mouse primary naïve CD4⁺ T cells for 6 days before analysis.
2. Flow cytometric (left) and statistical analysis (right) of IL‐4 and IL‐13 in Th2 cells derived from mouse primary naïve CD4⁺ T cells treated with or without JQ1 (500 nM).
3. qPCR analysis of Il4, Il5, and Il13 in mouse Th2 cells treated with or without JQ1.
4. ELISA analysis of IL‐4 secretion into the supernatant of mouse Th2 cells treated with or without JQ1.
5. Western blotting (left) and densitometry analysis (right) of Brd2, Brd3, and Brd4 in mouse naïve CD4⁺ T cells, Th0, Th1, Th2, Th17, and Treg cells.
6. Flow cytometric (upper) and statistical analysis (lower) of IL‐4 in mouse Th2 cells infected with sh‐Ctrl, sh‐Brd2, sh‐Brd3, or sh‐Brd4 lentivirus.

Data information: Mouse naïve CD4⁺ T cells were cultured in Th2 polarization condition and treated with or without inhibitors on Day 0 and were differentiated for 6 days before analysis, unless otherwise specified. All data represent mean ± SD and average of three independent experiments. Data are analyzed by Paired t test. *P < 0.05; **P < 0.01; and ***P < 0.001.

Source data are available online for this figure.