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. 2023 Feb 16;42(6):e112202. doi: 10.15252/embj.2022112202

Figure EV1. DSS leads to efficient induction of intestinal inflammation.

Figure EV1

  1. Representative H&E staining of colon histology and quantification on day 7 after DSS colitis induction (n = 3/group) from one independent experiment.
  2. Colon length measured after 1.5–2% DSS colitis regime on day 7 (n = 10–11/group).
  3. Spleen weight and mesenteric lymph node weight after 1.5–2% colitis regime on day 7 (n = 9–10/group).
  4. TNFα levels in serum were measured in wild‐type mice on day 7 after water and DSS treatment (n = 5/group).
  5. Absolute number of colonic CD45+ immune cells on day 7 post‐DSS treatment (n = 6–7/group).
  6. Frequency of CD11b+ myeloid cells, CD3+ T cells and CD19+ B cells in colon on day 7 post‐DSS treatment (n = 5–7/group).
  7. Colon length of mice upon DSS‐induced colitis treated with anti‐isotype or anti‐TNFα neutralizing antibody (n = 7/group).
  8. Body weight development upon DSS‐induced colitis of mice treated either with anti‐isotype or anti‐TNFα neutralizing antibody (n = 7/group).
  9. Tissue weight of mWAT or gWAT upon DSS‐induced colitis of mice treated either with anti‐isotype or anti‐TNFα neutralizing antibody (n = 7/group).

Data are represented as mean ± s.e.m. (A–E) Unpaired Student's t‐test. (G, I) Two‐way ANOVA. (H) Repeat‐measure two‐way ANOVA. **P < 0.01, ***P < 0.001, ****P < 0.0001.

Source data are available online for this figure.