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. 2023 Feb 16;42(6):e112202. doi: 10.15252/embj.2022112202

Figure 2. Loss of adipocyte autophagy exacerbates DSS‐induced colitis.

Figure 2

  1. Schematic of experimental design. Sex‐matched and age‐matched littermates were treated at 8–12 weeks of age with tamoxifen for 5 consecutive days before tissues were analyzed 14 days after the last tamoxifen administration (Steady State).
  2. Representative quantification of knock‐out efficiency measured on Atg7 transcript level by qRT‐PCR in purified primary visceral adipocyte at 2 weeks post‐tamoxifen treatment (n = 4–11/group).
  3. Representative immunoblot for LC3‐I and LC3‐II protein expression and quantification of LC3 conversion ratio (LC3‐II/LC3‐I) (n = 3/group).
  4. Schematic of experimental design. Sex‐matched and age‐matched littermates were treated at 8–12 weeks of age with tamoxifen for 5 consecutive days and DSS‐induced colitis was induced after a 2‐week washout phase (DSS Day 7).
  5. Body weight development upon DSS treatment (n = 25/group).
  6. Colon length after 2 weeks postdeletion (steady state; n = 14/group) and after DSS on day 7 (n = 18–22/group).
  7. Representative H&E staining images (10× magnification) of colon sections and quantification of histological score at steady state (n = 9/group) and DSS colitis (n = 18–22/group).
  8. Expression of pro‐inflammatory cytokines in colon tissues at 7 days post‐DSS induction (n = 18–22/group). Dotted line represents uninflamed controls.
  9. Absolute number CD45+ immune cells from colons at steady state (n = 13–14/group) or at 7 days post‐DSS induction (n = 18–22/group).
  10. Frequency of myeloid cell population in colon on day 7 post‐DSS induction (n = 18–25/group).
  11. Absolute number of Ly6C+ monocytes discriminated by the absence or presence of MHCII for infiltrating and inflammatory monocytes, respectively (n = 18–25/group).

Data are represented as mean ± s.e.m. (B, C, F–I) Unpaired Student's t‐test. (E) Two‐Way repeated measures ANOVA. (J, K) Two‐Way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Source data are available online for this figure.