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. 2023 Feb 16;42(6):e112202. doi: 10.15252/embj.2022112202

Figure EV2. Expansion of intestinal Treg populations is blunted in adipocyte autophagy‐deficient mice without affecting intestinal resolution.

Figure EV2

  1. Schematic of experimental design. Sex‐matched and age‐matched littermates were treated with DSS for 5 days and mice were sacrificed 14 days after start of DSS treatment.
  2. Colon length from noninflamed control mice (n = 8/group), adipocyte autophagy‐sufficient WT mice and adipocyte autophagy‐deficient mice (n = 12/group).
  3. Representative H&E staining images (10× magnification) of distal colon sections and quantification of histopathological score (n = 7–13/group).
  4. Frequency (left panel) and absolute number (right panel) of CD4+ FOXP3+ cells in the colon on day 14 post‐DSS treatment (n = 8–11/group).
  5. Frequency of peripheral and thymic Treg (pTreg and tTreg, respectively) cell populations in colon on day 14 post‐DSS treatment (n = 8–11/group).
Data are represented as mean ± s.e.m. (B–D) One‐way ANOVA. (E) Two‐way ANOVA. *P < 0.05, **P < 0.01, ****P < 0.0001. Source data are available online for this figure.