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. 2023 Feb 16;42(6):e112202. doi: 10.15252/embj.2022112202

Figure 5. Adipocyte autophagy loss activates NRF2‐EPHX1 pathway and alters intratissual oxylipin balance.

Figure 5

  1. Differential gene expression in visceral adipocytes from water‐treated WT and Atg7 Ad animals 2 weeks after tamoxifen treatment.
  2. Differential gene expression in visceral adipocytes from DSS‐treated WT and Atg7 Ad animals on day 7 post‐DSS treatment.
  3. Venn diagram of commonly regulated genes between Atg7‐deficient and Atg7‐sufficient adipocytes during water or DSS treatment.
  4. GSEA enrichment analysis between Atg7‐deficient and Atg7‐sufficient adipocytes during DSS treatment.
  5. Fold change expression of NRF2‐target genes in primary visceral adipocytes on day 7 after DSS induction from normalized counts of RNAseq dataset (n = 6/group).
  6. Representative immunoblot for NRF2 protein expression and quantification (n = 16–18/group).
  7. Transcriptional expression of Ephx1 and Ephx2 in visceral adipocytes on day 7 after DSS induction (n = 20–25/group).
  8. Representative immunoblot of EPHX1 and EPHX2 in gonadal adipose tissues on day 7 after DSS induction. Asterix indicating nonspecific band (n = 14–18/group).
  9. Schematic overview of cytochrome P450‐EPHX oxylipin pathway.
  10. Normalized fold change differences in epoxy fatty acid precursor fatty acids, docosahexaenoic acid (DHA), arachidonic acid (AA), and linoleic acid (LA) in mWAT and gWAT (n = 13–14/group).
  11. Normalized ratios of epoxy fatty acid to their corresponding diol fatty acid pairs in mWAT and gWAT (n = 6–8/group).

Data are represented as mean ± s.e.m. (E–H, L) Unpaired Student's t‐test. (J, K) Two‐way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Source data are available online for this figure.