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. Author manuscript; available in PMC: 2023 Mar 15.
Published in final edited form as: Nat Struct Mol Biol. 2022 Nov 11;29(11):1136–1144. doi: 10.1038/s41594-022-00855-y

Extended Data Fig. 5 |. CRISPR/Cas9-mediated deletion of distal CBS does not lead to uasTrx activation.

Extended Data Fig. 5 |

a, Genotype of distal site A edited Ahcyl1 clones shown in Fig. 2c. Predicted CTCF motif is highlighted in green. b, Left, CTCF ChIP-qPCR showing abrogation of CTCF binding at distal anchor A in clones distal site A rep1 and 2 shown in Fig. 2c (error bar: SEM; n = 2). Right, proximal CBS served as a control for ChIP efficiency (error bar: SEM; n = 2). c, Same as in (a) for Ahcyl1 distal site B. d, same as in (a) for Ahcyl1 distal site C. e, same as in (b) for Ahcyl1 distal site B and C. f, Genotype of distal site A and B edited Azi2 clones.