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(A) Time-dependent change in fluorescence spectra after treatment of βGlcNAc-CM-Rhod-P (10 μM) with ALP (100 nM) in 50 mM Tris buffer (pH 7.4) containing 1% DMSO (λex = 510 nm, Δt = 6 min). (B) Time-dependent fluorescence response of βGlcNAc-CM-Rhod-P (10 μM) to ALP (100 nM) in the absence and presence of either 50 μM PUGNAc or 1 mM Na3VO4 (λex = 510 nm, λem = 545 nm, Δt = 2 min).
