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. 2023 Mar 1;13:1142173. doi: 10.3389/fcimb.2023.1142173

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Copyright © 2023 Zhang, Li, Yang, Xiao, Dong, Lin, Liu and Shan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of erastin and Fer-1 on Vero cells infected by PEDV. (A) Relative quantification of PEDV for verification by RT-qPCR. The relative quantification of PEDV in Vero cells with different treatments by RT-qPCR was calculated by 2^-ΔΔCt method. (B) The Analyze the fluorescence intensity of virus-infected cells in each of experiments by using the ImageJ software. Error bars represent standard deviations of technical triplicates. *P<0.05, ***P < 0.001, n = 3 per group. (C) Vero cell with different treatments were collected at 10 hours, fixed, permeabilized, and stained with PEDV S protein (rabbit), then rabbit protein antibody (goat). DAPI was used for nuclear staining. Images were taken using a microscope. Scale bar = 100 μm.