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. 2023 Mar 1;13:1142173. doi: 10.3389/fcimb.2023.1142173

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Copyright © 2023 Zhang, Li, Yang, Xiao, Dong, Lin, Liu and Shan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Erastin affects the different stages of PEDV infection with Vero cell. (A) Vero cells or PEDV were treated with erastin in different ways before and after infection. Double-headed red arrows indicate the presence of erastin. The gray line indicates the PEDV infection. The experiments are identified in the text by the numbers on the left (M1-M10) and (N1-N6). Cell treatment time is calculated as hour post infection below. The relative quantity of RT-qPCR of PEDV in Vero cells treated with erastin was calculated by 2^-ΔΔCt method. (B–D) The relative quantity of PEDV in Vero cells at the stages of adhesion, invasion and replication was verified by RT-qPCR. The error bar represents the standard deviation * P<0.05, **** P<0.001, 3 cases in each group. ns, not significant.