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. 2023 Mar 1;13:1142173. doi: 10.3389/fcimb.2023.1142173

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Copyright © 2023 Zhang, Li, Yang, Xiao, Dong, Lin, Liu and Shan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Erastin affects the release of Vero cells infected with PEDV and the regulation of ferrotropis gene. (A) The relative quantity of PEDV in Vero cells at the release phase was verified by RT-qPCR. The left part (N1-N3) represents the virus in cells, and the right part (N4-N6) represents the virus in the supernatant. (B) Ferroptosis-suppressing pathways. NRF2, ACSL4 and GPX4 are key factors in the iron death signaling pathways of P53-NRF2 - (HO-1/NQO1), PUFA ACSL4 PUFA-PL and system Xc-GSH-GPX4. (C) The expression of NRF2, ACSL4 and GPX4 was determined by RT-qPCR, GAPDH was the loading control. Cell treatment time is calculated as per hours after infection, 2^-ΔΔCt method was used to calculate the relative quantity of RT-qPCR of PEDV in Vero cells treated with Erastin. The error bar represents the standard deviation *P<0.05, **P<0.01, ***P<0.001,3 cases in each group.