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Primers for real-time PCR.
| Primer name | Nucleotide sequence (5’-3’) | Product (bp) | GenBank accession number |
|---|---|---|---|
| PEDV N | F: ACTAATAAAGGGAATAAGGACCAG | 207 | OP894120 |
| R: GTTAGTGGGTTCAGTCTTTGC | |||
| ACSL4 | F: AATAGACATCCCTGGAGCAGATACT | 119 | NM_001318509.2 |
| R: GCTGCATTTCATTTTCTTCACTTAG | |||
| NRF2 | F: TCCAGTCAGAAACCAGTGGAT | 109 | HM446346.1 |
| R: GAATGTCTGCGCCAAAAGCTG | |||
| GPX4 | F: CAGTGAGGCAAGACCGAAGTGAAC | 125 | NM_001039847.3 |
| R: TTACTCCCTGGCTCCTGCTTCC | |||
| GAPDH | F: CCCACTCCTCCACCTTTGAC | 113 | NM_002046.7 |
| R: TCCACCACCCTGTTGCTGTAGC |
