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. Author manuscript; available in PMC: 2023 Mar 15.
Published in final edited form as: Matrix Biol. 2022 Aug 22;112:132–154. doi: 10.1016/j.matbio.2022.08.009

Figure 4. HMCN1 and NID2 proteins display partially overlapping localization at basement membranes of mouse embryos.

Figure 4.

(A-C) Immunofluorescence analysis of skin at the level of the whisker follicle of embryo at E14, using antibodies directed against HMCN1 (red) and NID2 (green); the section was counterstained with DAPI to label nuclei (blue). Merged (A,B,C) as well as single NID2 (A’,C’) and single HMCN1 (A”,C”) channels are shown. (A,C) show magnifications of regions boxed in B. In apical regions of the whisker follicle, dermal HMCN1 protein has accumulated underneath the BM, largely, but not completely, separated from NID2 located within the BM (A,A’,A”,C,C’,C”; indicated by arrows labelled with s). In contrast, in deeper regions of the whisker follicle, HMCN1 is largely located within the BM, co-localizing with NID2 (insets C,C”,C”; indicated by arrows labelled with c). (D) Overview of ultrastructural immunolocalization of HMCN1 protein on skin sections of P14 mouse. (E) Higher magnification reveals clusters of HMCN1 signals underneath the BM. (F-H) Consecutive transverse sections of musculus gastrocnemius from wild-type adult mouse immunofluorescently labelled with antibodies against HMCN2 (F), NID2 (G) and LAMγ1 (H). HMCN2, NID2 and LAMγ1 display similar distributions along endomysial BMs of muscle fibers. Abbreviations: bm, basement membrane; c, co-localized; de, dermis; hf, hair follicle; s, separated; wi, whisker.