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Dissociation constants (KD values) between mouse HMCN1 fragments and full-length or G3 domains of mouse NID1 and NID2
| HMCN1 fragments | NID2 | NID2-TG3 | NID1 | NID1-TG3 |
|---|---|---|---|---|
| TSR6-G2F-EGF1–2 | 160 nM | 176 nM | -- | -- |
| G2F | > 3 μM | > 3 μM | -- | -- |
| G2F-EGF1 | > 3 μM | > 3 μM | -- | -- |
| G2F-EGF1–2 | 200 nM | 185 nM | -- | -- |
| G2F-EGF1–3 | 210 nM | 170 nM | -- | -- |
| G2F-EGF1–4 | 193 nM | 180 nM | -- | -- |
| G2F-EGF1–5 | 20 nM | 30 nM | 560 nM | 585 nM |
| G2F-EGF1–5 (no Ca2+) | ND | -- | ND | -- |
| EGF1–5 | 500 nM | 620 nM | -- | -- |
--: Not determined; ND: not detectable
