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. 2023 Jan 22;10(8):2205668. doi: 10.1002/advs.202205668

Figure 6.

Figure 6

PKR is a functional mediator for circWDR37. a) Representative images and quantified results of the colony formation assay in si‐PKR or siSCR transfected HONE1 cells with cisplatin or gemcitabine treatment. b) Representative images and quantified results of SA‐β‐gal staining si‐PKR or siSCR transfected HONE1 cells with cisplatin or gemcitabine treatment. c) Western blots analysis of p16 and cyclin D1 in si‐PKR or siSCR transfected HONE1 and S18 cells with cisplatin or gemcitabine treatment. d) Representative images and quantified results of the transwell migration and invasion assays in si‐PKR or siSCR transfected HONE1 cells with cisplatin or gemcitabine treatment. e) Representative images and quantified results of transwell migration and invasion assays in the HONE1 cells co‐transfected with si‐circWDR37 or siSCR together with PKRT446DT451D‐Flag or empty vector. f) Representative images and quantified results of SA‐β‐gal staining in the HONE1 cells co‐transfected with si‐circWDR37 or siSCR together with PKRT446DT451D‐Flag or empty vector. g) Luciferase assay of NF‐κB activity in HONE1 and S18 cells co‐transfected with si‐circWDR37 or siSCR together with PKRT446DT451D‐Flag or empty vector. h) Western blots analysis of the indicated proteins in HONE1 and S18 cells co‐transfected with si‐circWDR37 or siSCR together with PKRT446DT451D‐Flag or empty vector. Mean (n = 3) ± s.d. (Data were analyzed by (a, b, d) one‐way ANOVA with Dunnett's post‐hoc test and (e–g) one‐way ANOVA with Tukey's post‐hoc test). p‐value < 0.05 indicates statistical significance. N.S. indicates no significance. Scale bar, 200 µm.