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. 2023 Feb 20;15(3):evad029. doi: 10.1093/gbe/evad029

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© The Author(s) 2023. Published by Oxford University Press on behalf of Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Inactivation of crtIBY in A. limacinum. (A) Agar plate streaks of WT with natural carotenogenic pigmentation relative to the two pigment-less crtIBY KOs (32 and 33). (B) Annotated genome maps (SnapGene) generated from nanopore sequencing of the A. limacinum (WT) and two KOs (32 and 33) reveal an intact crtIBY locus in WT and disrupted, yet successful integration of shble in the crtIBY open reading frame in the two KOs. Sequences indicate a double homologous recombination event having occurred in 32, while a triple tandem repeat integration event occurred in 33. Both integration events resulted in a nonfunctional form of the CrtIBY protein.