FIG 2.
Copy number gain of the sigB locus in JE2 replicate 3. (A) Plot of estimated copy number for regions across the JE2 reference genome. (B) Enlargement of the region affected by copy number gain. Locations of genes carried in the forward orientation (orange dots) and reverse orientation (gray dots) are indicated along the central axis, with the location of sigB (reverse orientation) highlighted in red. (C) Confirmation of copy number gain by real-time PCR. Analysis was performed using primers designed upstream and downstream of the presumptive amplification and within both ends of the amplification. DNA copy number was estimated by the ΔΔCT method, using an uninvolved region of the genome as a comparator. Error bars indicate standard deviation from three separate measurements.