TABLE 1.

Gene mutations associated with increased biofilm formation in vitro

Pangenome gene identifier	Common gene name	Gene function	Mutant count per selected strain (total replicates)
			Newman (n = 5)	JE2 (n = 5)	N315 (n = 3)	ATCC 29213 (n = 5)
SAUPAN000916000	sbnC	IucA/IucC family siderophore biosynthesis protein	1	0	0	0
SAUPAN005823000	spdC	Lysostaphin resistance protein A	0	2	3	0
SAUPAN006410000	icaR	Biofilm operon icaADBC HTHa-type negative transcriptional regulator	2	5	0	0
SAUPAN005927000	narH	Nitrate reductase subunit beta	1	0	0	0
SAUPAN003492000	rpoZ	DNA-directed RNA polymerase subunit omega	0	1	0	0
SAUPAN004777000		DUF445 domain-containing protein	1	0	0	0
SAUPAN003350000		Hypothetical protein	1	0	0	0
SAUPAN003016000		Nitronate monooxygenase	0	0	0	1
SAUPAN003553000	codY	GTP-sensing pleiotropic transcriptional regulator	3	5	3	3
SAUPAN005440000	manA	Mannose-6-phosphate isomerase	0	0	1	0
SAUPAN001024000	argC	N-Acetyl-gamma-glutamyl-phosphate reductase	0	0	1	0
SAUPAN006300000		Amino acid permease	0	0	0	1
SAUPAN003545000	dprA	DNA processing protein	1	0	0	0
SAUPAN002478000		HAD family hydrolase	0	0	0	1
SAUPAN006252000	gbaA	TetR family transcriptional regulator	0	0	1	0
SAUPAN001228000		5′-Nucleotidase, lipoprotein e(P4) family	0	1	0	0
SAUPAN006369000	isaB	Immunodominant antigen B	0	0	0	1
SAUPAN002607000		ABC transporter ATP-binding protein	1	0	0	0
SAUPAN002583000	fruB	1-Phosphofructokinase	1	0	0	0
SAUPAN000959000		Bifunctional metallophosphatase/5′-nucleotidase	1	0	0	0
SAUPAN000382000	dusB	tRNA-dihydrouridine synthase	0	0	1	0
SAUPAN005009000	eap/map	MAP domain-containing protein	2	0	0	0
SAUPAN000011000	gyrB	DNA gyrase subunit B	0	1	0	0

^aHTH, helix-turn-helix.