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. Author manuscript; available in PMC: 2023 Mar 15.
Published in final edited form as: J Vis Exp. 2022 Jun 7;(184):10.3791/64056. doi: 10.3791/64056

Figure 1: Schematic of levamisole assay preparation and implementation.

Figure 1:

24-well NGM plates are poured; 2 days later, OP50 Escherichia coli is seeded into the wells. C. elegans are synchronized through bleach prep, and first larval stage (L1) animals for each strain to be assayed (wild type, black; unc-63(x26), magenta; lev-10(x17), green; unc-49(e407), blue) are pipetted into the wells according to a predetermined plate map the following day. After 3 days of growth at 20 °C, or when the animals reach adulthood, 0.4 mM levamisole in M9 buffer is added to each well, and the number of moving animals is counted every 5 min for 1 h.