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. 2023 Mar 13;11(3):e006522. doi: 10.1136/jitc-2022-006522

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© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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An Akt-resistant form of PGC-1α and NT-PGC-1α improve the mitochondrial fitness of human CAR T cells in vitro. (A) Schema of different versions of PGC-1α, (B) real-time-PCR analysis of target genes from HEK293T cells transduced with different versions of PGC-1α, (C) MitoTracker staining of CAR-T cells carrying the PGC-1α construct or the NT-PGC-1α construct in vitro, (D) Seahorse analysis of CAR T cells carrying the PGC-1α or the NT-PGC-1α construct in vitro. The MitoFM experiments were repeated with five donors. The Seahorse analysis is representative of three donors each. Statistics are paired t-test (C) and Wilcoxon rank-sum test (D). *p<0.05, **p<0.01. CAR, chimeric antigen receptors; PGC-1α, PPAR gamma coactivator 1α.