Figure 5.

Becn1^F121A mice contain an altered gut microbiota

16S rRNA sequencing was performed to characterize gut microbiota composition.

(A and B) (A) PCoA of fecal microbiota β diversity based on weighted UniFrac and (B) Bray-Curtis dissimilarity in mice housed at the Bar-Ilan University.

(C) α diversity comparison based on richness and evenness.

(D) Relative taxonomic composition.

(E) Cladogram depicting LEfSe analysis of differently abundant bacteria in wild-type and Becn1^F121A mice.

(F) Relative abundance of Akkermansia muciniphila in mice housed at the Bar-Ilan University.

(G) Principal coordinates analysis (PCoA) of fecal microbiota β diversity based on weighted UniFrac on mice housed at UTSW.

(H) Relative abundance of Akkermansia muciniphila in mice housed at UTSW.

(A, B, and F–H) Each symbol represents a mouse. (D) Each column represents a mouse. ^∗∗∗p < 0.001; ^∗∗∗∗p < 0.0001; (C, F and H) Student’s t test. WT, wild type; F121A, Becn1^F121A; UTSW, UT Southwestern.