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. 2023 Feb 1;127(5):1138–1143. doi: 10.1021/acs.jpcb.2c08270

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Annealing of ribonuclease. The percentage of structured protein is plotted versus the temperature. The percentage of structured protein was calculator using the gromacs tool “gmx do dssp”, which uses the DSSP algorithm.^25,26 The blue line is SPC/E (H₂O), and the red line is SPCE-HW (D₂O). Error bars are not reported as they are too small to be visible in the figure (i.e., below 1%).