Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 1;14(2):529–547. doi: 10.14336/AD.2022.0826

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

copyright: © 2022 Shen et al.

this is an open access article distributed under the terms of the creative commons attribution license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Figure 5. — Hypoxia-induced Hsa_circ_0000566 stabilizes HIF-1α by attenuating interactions between Von Hippel—Lindau (VHL) and HIF-1α. (A) The interaction between VHL and Hsa_circ_0000566, assessed by performing a RIP assay. Results are reported as mean ± standard deviation (SD), *p < 0.05, n = 3. (B) Interaction between VHL and Hsa_circ_0000566, identified by conducting pulldown assays. (C) Effects of Hsa_circ_0000566 silencing and Hsa_circ_0000566 overexpression on VHL protein and mRNA expression, identified by performing western blotting and quantitative real-time polymerase chain reaction (qRT-PCR). (D) Immunoprecipitation experiment revealing VHL-HIF-1α interaction that is inhibited by Hsa_circ_0000566 overexpression. (E) Sequential coimmunoprecipitation assay, performed to determine whether Hsa_circ_0000566 could simultaneously bind VHL and HIF-1α.