Optimization of different GelMA conditions for the semi-opened culture system. a. The follicle was cultured in different GelMA conditions on day 2. The scale bar was 200 μm. b. The follicle was cultured in different GelMA conditions on day 10. The scale bar was 200 μm. c. The follicle diameter in 10% and 15% GelMA conditions was larger than that in 5% GelMA, n > 11, the error bar stands for standard error of the mean, the p-value passed two-way ANOVA test, ns: p > 0.05; ****: p < 0.0001. d. The follicle size distribution in different GelMA conditions, the p-value passed the Chi-square test, ****: p < 0.0001. e. The fitting curve of elastic modulus distribution of mouse ovary and GelMA 60. f. The fitting curve of elastic modulus distribution of mouse ovary and GelMA 90.