Fig. 8.

The MCO is a promising in vitro model for chemical test. a. The ROS level in 2D, MCO, and 3D culture systems, the scale bar is 100 μm; b. The fluorescent intensity of ROS level in 2D, MCO, and 3D culture systems, n > 4; c. The ROS level was reduced by melatonin within MCO, the scale bar is 100 μm; d. The fluorescent intensity of ROS level within MCO, n > 4; e. The time-lapse record of the ovulation process under the treatment of H₂O₂ or H₂O₂ plus melatonin, the scale bar is 100 μm; f. The perimeter variation of follicles during the ovulation in H₂O₂ or H₂O₂ plus melatonin condition, 0h was set as basal level, n > 4; g. the proportion of GV, GVBD, and MII oocyte in different conditions.