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. 2023 Mar 2;11:1138504. doi: 10.3389/fcell.2023.1138504

FIGURE 2.

FIGURE 2

Maturation-dependent expression of renal drug transporters in kidney organoids, and comparison with human kidneys and HKC-8 cultured cells. (A) Heatmap for RNA-seq of drug transporters icluding OAT1, OAT3, OCT2, and PMAT in undifferentiated ES cells (day 0), nephron progenitor cells (NPC, day 8), and kidney organoids (day over 21). n = 3 to 9. (B) qPCR of OAT1, OAT3, and OCT2 in undifferentiated ES cells, kidney organoids (day 21), and HKC8 cells. n = 3 to 9. *p < 0.05. (C) Gating of dissociated organoids by LTL. Even though only 22.60% of dissociated cells from organoids showed LTL positive, the mRNA level of OCT2 from organoids was comparable to that of HKC-8. (D) Localization of OAT1, OAT3, and OCT2 in kidney organoids (day 21). IF of podocalyxin (PODXL, green), LTL (cyan), OAT1 (red), OAT3 (red), and OCT2 (red). Podocytes did not express OAT1, OAT3, and OCT2 in kidney organoids. (E–G) Immunofluorescence (IF) of LTL (cyan), OAT1 (red), OAT3, and OCT2 in kidney organoids (day 51), human kidney samples, and HKC-8 cells. Scale bar: 50 μm.