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. 2023 Mar 2;11:1138504. doi: 10.3389/fcell.2023.1138504

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Copyright © 2023 Susa, Kobayashi, Galichon, Matsumoto, Tamura, Hiratsuka, Gupta, Yazdi, Bonventre and Morizane.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of the segment-specific injury by Perceval HR organoids. (A) Representative BF image and IF image of PODXL (red), LTL (cyan), and CDH1 (yellow) in 2D organoids. Scale bars: 200 μm. (B) Representative images for quantification of ATP/ADP ratio matched in position with the BF and IF images for segment identification in 2D organoids exposed to 5 μM cisplatin. Scale bars: 200 μm. (C) Detection of ATP/ADP ratio of each segment in 2D reporter organoids treated with hypoglucose, 5 and 50 μM cisplatin for 24 h n = 4 to 15. *p < 0.05.