Table 1.
Commercially available antibodies used for immunofluorescence microscopy.
| Antibody | Manufacturer | Catalog Number | Dilution | Species |
|---|---|---|---|---|
| Pax7 | DSHB | Pax7 | 1:50 | Mouse IgG1 |
| Dystrophin | Thermo | RB-9024 | 1:100 | Rabbit |
| Type IIa fibers | DSHB | SC-71 | 1:100 | Mouse IgG1 |
| Type I fibers | DSHB | BA-F8 | 1:100 | Mouse IgG2b |
| Type IIb fibers | DSHB | BF-F3 | 1:100 | Mouse IgM |
| Embryonic myosin heavy chain | DSHB | F1.652 | 1:50 | Mouse IgG1 |
| CD31 | BioRad | MCA2388 | 1:200 | Rat |
| Dystrophin | Abcam | ab3149 (discontinued) | 1:100 | Mouse IgG1 |
| Perilipin | Cell Signaling Technologies | 9349S | 1:200 | Rabbit |
| PDGFRα | Cell Signaling Technologies | 3174S | 1:800 | Rabbit |
| Myogenin | DSHB | F5D | 1:50 | Mouse IgG1 |
| Myosin heavy chain | DSHB | A4.1025 | 1:200 | Mouse IgG2a |