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. 2023 Mar 15;14:1439. doi: 10.1038/s41467-023-37199-0

Fig. 3. Flt3-expressing fetal HSC-derived multipotent progenitors give rise to adult testicular macrophages.

Fig. 3

a–h Representative images (n = 3 independent gonads) of Flt3-cre; Rosa-Tomato testes at E14.5 (a), E16.5 (b), E18.5 (c), P7 (d), P30 (e, f), and P90 (g, h). Arrowheads denote Tomato-expressing F4/80+ macrophages and arrows denote Tomato-expressing F4/80-negative cells. Thin scale bar, 100 μm; thick scale bar, 25 μm. i–k Graphs showing quantification of percent Tomato-expressing F4/80+ macrophages (i), number of Tomato-expressing F4/80-negative cells per unit area at E14.5 (n = 3 independent gonads), E16.5 (n = 3), E18.5 (n = 5) or P7 (n = 3) (j), and percent Tomato-expressing interstitial (CSF1R+) and peritubular (MHCII+) macrophages at P30 (n = 3) and P90 (n = 4) (k) in Flt3-cre; Rosa-Tomato testes. Data are shown as mean +/− SD. *P < 0.05; **P < 0.01; ***P < 0.001 (two-tailed Student’s t-test). Exact P values are provided in the Source Data file.