Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 15;14:1439. doi: 10.1038/s41467-023-37199-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a–l Representative images (n = 3 independent gonads) of control (a, c, e, g, i, k) and Amh-cre; Rosa-DTA (b, d, f, h, j, l) testes at various fetal stages. Thin scale bar, 100 μm; thick scale bar, 25 μm. m, n Graphs showing number (n = 3 independent gonads) of F4/80⁺ cells (m) or number of CD45⁺ IBA1-negative cells (n) per unit area of gonadal interstitium at E14.5, E16.5, or E18.5 in control versus Amh-cre; Rosa-DTA fetal testes, as determined by stereology-based cell counts. o Representative flow cytometry analyses of E18.5 control (left) and Amh-cre; Rosa-DTA (right) fetal testes for CD45⁺ cells (top) and for F4/80-hi CD11b-hi (macrophages; red gate) versus F4/80-lo CD11b-hi cells (monocytes and other myeloid cells; blue gate) (bottom). p–s Graph showing flow-cytometric-based quantification of percent CD45⁺ cells among live total gonadal cells (p), number of CD45⁺ cells per 1 × 10⁵ live total gonadal cells (q), percent F4/80-hi CD11b-hi and F4/80-lo CD11b-hi cells among CD45⁺ cells (r), and number of F4/80-hi CD11b-hi and F4/80-lo CD11b-hi cells per 1 × 10⁵ live total gonadal cells (s) for E18.5 control (n = 3) versus Amh-cre; Rosa-DTA (n = 4) fetal testes. Data are shown as mean +/– SD. *P < 0.05 (two-tailed Student’s t-test). Exact P values are provided in the Source Data file.