Figure 5.

SkQ1 prevents increase in the level of proinflammatory cytokines IL-6 and TNF-α induced by injection of mitochondria. Mitochondria were injected as in Fig. 4. (A,B) SkQ1 (1.5 μmol/kg) was administered intraperitoneally daily for 5 days prior to mitochondrial injection. (B) Mitochondria isolated as in Fig. 4 and (A) were compared with mitochondria isolated in the absence of BSA, which have ΔΨ below 150 mV. Succinate was added to mitochondria prior to injection as in Fig. 4. (C) Mitochondria isolated as in Fig. 4 and (A) were preincubated with 0.5 mM malonate, 2 μM rotenone or 1 nM SF6847 in the presence of 5 mM succinate prior to injection. Each of the groups contained 6 mice. *p < 0.05. Each of the groups contained 8 mice. In (A) the number of mice in each group was 6, and in (B,C) in each group 8. *p < 0.05, **p < 0.01, ***p < 0.001.