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Confocal fluorescence microscopy of autophagy puncta. HEK293T cells were transfected with GFP-LC3 plasmid alone or with pcDNA3.1-HA-TgSTING plasmid for 24 h. For positive control, 100 nM rapamycin was added into cells for another 24 h. DAPI was used to stain cell nuclei. Scale bar = 25 μm.
