Figure 3.

RSV antibodies modify type I IFN gene expression. RSV was pre-incubated with 10 μg/mL mAb for 1 h at 37°C then added onto MLE-15 cells for 24 h. Twenty-four hours post infection, expression of (a) IFNA1, (b) IFNB, and (c) ISG15 genes was determined in MLE-15 cells. Bars represent the mean ± SEM of gene expression fold change using ΔΔCt PCR method normalized to ACTB expression and mock-infected cells. *p < 0.05 as determined by one-way ANOVA with Dunnett’s post hoc test compared with isotype control. Experiments were individually repeated at least twice with technical replicates.