Figure 4.
RSV antibodies modify IFNλ2/3 responses. (a) RSV Line19 F was pre-incubated with 10 μg/mL mAb for 1 h at 37°C, then added onto MLE-15 cells for 24 h, and supernatant was assayed for IFNλ2/3. (b) The BALB/c mice were i.p. treated prophylactically with 1 mg/kg of indicated mAb 24 h before i.n. infection with 106 PFU Line19 F. Twenty-four hours post infection, BALF was collected and assayed for IFNλ2/3. Concentration of IFNλ2/3 was determined by ELISA and quantified using a standard curve of IFNλ2/3. Bars represent the mean ± SEM of IFNλ2/3 (pg/mL). *p < 0.05 as determined by one-way ANOVA with Dunnett’s post hoc test compared with isotype control. For in vitro, bars represent the mean of three independent studies with technical replicates. For in vivo, bars represent mean of three independent assays with technical replicates from one experiment of 5 mice/group.