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. 2023 Mar 2;14:1099799. doi: 10.3389/fimmu.2023.1099799

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Copyright © 2023 Yamaguchi, Maeshige, Yan, Ma, Uemura, Matsuda, Nishimura, Hasunuma, Kondo, Fujino and Yuan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Extracellular vesicle (EV) characterization. (A) The presence of CD63 on isolated EVs was verified by flow cytometry. (B) CD63 was absent in particles treated with the isotype control. (C) Western blot analysis of CD63. (D) Size distribution of isolated EVs was measured by tunable resistive pulse sensing. n = 3.