KEY RESOURCES TABLE.

REAGENT or RESOURCE	SOURCE	IDENTIFIER

Antibodies

Mouse anti-LAMPI (H4A3)	Santa Cruz Biotechnology	Cat#sc-20011; RRID:AB_626853
Rat anti-LAMPI (1D4B)	Santa Cruz Biotechnology	Cat#sc-19992; RRID:AB_2134495
Mouse anti-cathepsin D (C-5)	Santa Cruz Biotechnology	Cat#sc-377124
Mouse anti-Vps41 (D-12)	Santa Cruz Biotechnology	Cat#sc-377118; RRID:AB_2687987
Mouse anti-Arl8a/b (H-8)	Santa Cruz Biotechnology	Cat#sc-398635
Mouse anti-Vps39 (C-5)	Santa Cruz Biotechnology	Cat#sc-514762; RRID:AB_2687985
Mouse anti-CD71 (H68.4)	Santa Cruz Biotechnology	Cat#sc-65882; RRID:AB_1120670
Rabbit anti-cathepsin D	Sigma Aldrich	Cat#219361; RRID:AB_10683361
Rabbit anti-lyspersin	Abcam	Cat#ab247064
Rabbit anti-Rab7a	Abcam	Cat#ab137029; RRID:AB_2629474
Rabbit anti-myrlysin	Proteintech	Cat#17169-1-AP; RRID:AB_2137150
Rabbit anti-Rab2	Proteintech	Cat#15420-1-AP; RRID:AB_2176874
Rabbit anti-Arl8b	Proteintech	Cat#13049-1-AP; RRID:AB_2059000
Rabbit anti-Vps39	Proteintech	Cat#16219-1-AP; RRID:AB_11043179
Rabbit anti-Vps33a	Proteintech	Cat#16896-1-AP; RRID:AB_2214916
Rabbit anti-EEA1	Thermo Scientific	Cat#MA5-14794; RRID:AB_10985824
Mouse anti-Histidine tag	Biorad	Cat#MCA 1396; RRID:AB_322084
Rabbit anti-Rab7a	T. Watts (University of Toronto, Toronto, ON, Canada), Bertram et al.⁵⁸	N/A

Chemicals, peptides, and recombinant proteins

Ferrofluid (EMG508)	Ferrotec	N/A
Polybead Carboxylate 1.0 micron microspheres	Polysciences	Cat#08226-15
Polybead Carboxylate 2.0 micron microspheres	Polysciences	Cat#18327
ATTO488 NHS-ester	ATTO-TEC	Cat#AD488-35
Brefeldin A	Santa Cruz Biotechnology	Cat#sc-200861
N-ethylmaleimide	Sigma Aldrich	Cat#E3876
Nocodazole	Sigma Aldrich	Cat#M1404
Dynarrestin	Sigma Aldrich	Cat#SML2332
Hoechst 33342	Immunochemistry technologies LLC	Cat#639
Amino dextran 10 kDa	Invitrogen	Cat#D1860
Syto13	Invitrogen	Cat#S7575
Lysotracker Red DND-99	Invitrogen	Cat#L7528
Opti-mem I Reduced Serum Medium	Gibco	Cat#31985070
Dulbecco’s modified Eagle medium (DMEM)	Gibco	Cat#31053-028
Sodium pyruvate	Gibco	Cat#11360-070
Penicillin-Streptomycin 10000 U/mL (PenStrep)	Gibco	Cat#15140-122
Fetal bovine serum Standard (FBS)	Pan Biotech	Cat#P30-3306

Experimental models: Cell lines

HeLa WT cells	Pu et al.¹⁴	N/A
HeLa Arl8b KO cells	Guardia et al.¹⁹	N/A
HeLa Arl8a Arl8b KO cells	Keren-Kaplan et al.¹⁸	N/A
HeLa Vps41 KO cells	Anderson et al.⁵⁹	N/A
HeLa myrlysin KO cells	Pu et al.¹⁴	N/A
HeLa diaskedin KO cells	Jia et al.²⁰	N/A
HeLa Rab7a KO cells	This paper	N/A
J774E macrophage-like cells	P.D. Stahl (Washington University, St. Louis, USA), Fiani et al.⁶⁰	N/A

Oligonucleotides

Mission siRNA universal negative control #1	Sigma Aldrich	Cat#SICO01-10NMOL
Custom siRNA, human Rab2a (5′–3′): GAAGGAGUCUUUGACAUUA[dT][dT]–3′	This paper, sequence derived from Lörincz et al.²⁷	N/A

Custom siRNA, mouse Vps41 (5′–3′): UUGGCUUAUUUAGUUAGCAAA[dT][dT]	This paper	N/A
Custom siRNA, mouse Rab7a (5′–3′): CCAUCAAACUGGACAAGAA[dT][dT]–3′	This paper	N/A
Primer Arl8b forward (5′–3′) TAAGCACTCGAGATGCTGGCGCTC ATCTCC–3′	This paper	N/A
Primer Arl8b reverse (5′–3′) TGCTTACCGCGGGCTTCTTCTAGAT TTTGAATGCTGA	This paper	N/A
SgRNA human Rab7a: UGAAUUUCUUAUUCACAUAC	Synthego	N/A

Recombinant DNA

pET45b(+)-hArl8b T34N	M. Sharma (Department of Biological Sciences, Indian Institute of Science Education and Research	N/A
	Mohali, Punjab, India), Khatter et al.⁷
pET45b(+)-hArl8b Q75L	M. Sharma, Khatter et al.⁷	N/A
pGEX4T3-hArl8b	M. Sharma, Khatter et al.⁷	N/A
pmCherry-N1-hArl8b	This paper	N/A
pmCherry-N1	D. Fürst (Cell Biology Institute, University of Bonn, Germany)	N/A
peGFP-C1-Rab7a T22N	B. van Deurs (University of Copenhagen, Copenhagen, Denmark). Bucci et al.⁸	N/A

Software and algorithms

ImageJ	Schneider et al.⁶¹	https://imagej.nih.gov/ij/
Image J plugin JACoP	Bolte and Cordelieres⁶²	https://imagej.nih.gov/ij/plugins/ track/jacop.html
Image J plugin oval profile	Bill O’Connell	https://imagej.nih.gov/ij/plugins/ oval-profile.html
GraphPad Prism 9	GraphPad Software	https://www.graphpad.com/ scientific-software/prism/
MathCad 14	PTC	N/A