Figure 2.

Positional and base-specific effects on translation initiation. (A) Contribution of variable mRNA positions to the observed rTR variance. The relative sum of squares calculated by ANOVA with each position as covariate is displayed. (B) Base-specific effects of the randomised positions. Displayed effects are log2-transformed fold changes (log₂ FC) of the mean rTR of variants with a given base at the respective position over the mean rTR of variants with any other base permitted at that position. Positive and negative values correspond to translation-increasing or -decreasing effects, respectively. Crossed boxes indicate unvaried bases. (C) Enrichment of bases amongst strong variants. The log₂ FC of a base's relative occurrence amongst strong variants (rTR ≥ 0.5) over its relative occurrence amongst weak variants (rTR < 0.5) is displayed.