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. 2023 Jan 9;51(5):2011–2032. doi: 10.1093/nar/gkac1232

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Functional validation of yeast Elongator. (A) Cryo-EM map and atomic model of the apo ScElp456 subcomplex. (B) Front view on the atomic model of yeast Elongator (left). Atomic model in surface representation at different orientations with a position of selected mutations represented in sticks (point mutation) and cartoon (deletion or truncation). Colouring corresponds to the affiliation of the particular position to the given subunit. (C) Phenotypic analyses of various yeast strains carrying variations in Elp1, Elp3, Elp4, Elp5 and Elp6 using zymocin resistance assays.