Table 6.

DynaMut2 results for positively selected sites from proteins E, M, and N.

Protein	Site	Mutation	Frequencya	Predicted Stability Change (ΔΔGStability)
M	94	S → G	0.45%	−0.19 kcal/mol (Destabilizing)
M	94	S → I	0.09%	0.65 kcal/mol (Stabilizing)
M	125	H → L	0.04%	1.83 kcal/mol (Stabilizing)
M	125	H → Q	0.04%	0.52 kcal/mol (Stabilizing)
M	125	H → Y	0.18%	2.04 kcal/mol (Stabilizing)
N	63	D → G	18.53%	0.08 kcal/mol (Stabilizing)
N	63	D → Y	0.04%	1.0 kcal/mol (Stabilizing)
N	151	P → L	0.22%	−0.43 kcal/mol (Destabilizing)
N	151	P → S	0.09%	−0.17 kcal/mol (Destabilizing)
N	265	T → I	0.04%	−0.32 kcal/mol (Destabilizing)
N	289	Q → L	0.04%	0.42 kcal/mol (Stabilizing)
N	289	Q → H	0.22%	−0.38 kcal/mol (Destabilizing)
N	296	T → I	0.09%	−0.75 kcal/mol (Destabilizing)
N	362	T → I	0.18%	−0.12 kcal/mol (Destabilizing)
N	362	T → K	0.09%	−0.09 kcal/mol (Destabilizing)

^aMutation frequency in the multiple sequence alignment (n = 2,240 sequences).