Fig. 2.

Locating specific mutations in the MprF protein. A dual-purpose enzyme called the S. aureus multiple peptide resistance factor (MprF) aids positively charged L-PG in moving from the inner to outer leaflet of the CM. A core bifunctional domain, a C-terminal L-PG synthase domain, and an N-terminal L-PG translocase (flippase) domain make up MprF. This domain connects the L-PG synthase and translocase domains at either end. Compared to past efforts, the projected MprF topology was different (Bayer et al., 2014).