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HPLC analysis of carotenoids in E. coli expressing CsBCH1 or CsBCH2. (A–F) pHIS8 (A), pHIS8-CsBCH1 (B), pHIS8-CsBCH2a (C), pHIS8-CsBCH2b (D), pHIS8-CsBCH2a-s (E), or pHIS8-CsBCH2b-s (F) was co-transformed into E. coli BL21(DE3) cells with pACCAR16∆crtX. Reverse-phase HPLC was used to separate and determine carotenoids with spectra extracted at 450 nm. AU, absorbance unit; peak 1, β-carotene; peak 2, β-cryptoxanthin; peak 3, zeaxanthin.
