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. 2022 Dec 30;10(3):uhac290. doi: 10.1093/hr/uhac290

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nanjing Agricultural University.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Carotenoid accumulation and carotenogenic gene expression levels in CsBCH2-overexpressing transgenic lines of citrus callus Rm. (A) Relative expression levels of CsBCH2 (left) and total content of carotenoid (right) in transgenic calli Rm. (B) Composition and content of carotenoid in transgenic calli Rm. (C) Expression patterns of carotenogenic genes in transgenic lines. (D) Phenotypes of transgenic line callus Rm. (E) Schematic of general pathways of carotenoid biosynthesis in citrus. Upregulated genes in blue; downregulated genes in orange. Data are expressed as mean ± standard error for triplicate samples. Asterisks above the bars indicate statistically significant differences compared with EV (Student’s t-test): ^***P < .001.