KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
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| Antibodies | ||
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| Mouse monoclonal ant-Phospho-STAT3 (Tyr705) (M9C6) | Cell Signaling Technology | Cat# 4113 RRID: AB_2198588 |
| Rabbit monoclonal ant-Phospho-STAT3 (Ser727) | Cell Signaling Technology | Cat# 9134 RRID: AB_331589 |
| Mouse monoclonal anti-STAT3 (124H6) | Cell Signaling Technology | Cat# 9139 RRID: AB_331757 |
| Rabbit polyclonal anti-AKT | Thermo Fisher Scientific | Cat# PA5-96351 RRID: AB_2808153 |
| Rabbit polyclonal anti-Phospho-AKT (Ser 124) | Thermo Fisher Scientific | Cat# PA5-105161 RRID: AB_2816634 |
| Rabbit polyclonal anti-Phospho-Src (Tyr418) | Millipore | Cat# 07-909; RRID: AB_568805 |
| Rabbit polyclonal anti-Src | Cell Signaling Technology | Cat# 2108; RRID: AB_331137 |
| Rabbit anti-Phospho-p70 S6 Kinase (Thr421/Ser424) | Cell Signaling Technology | Cat# 9204 RRID: AB_2265913 |
| Rabbit anti-p70 S6 Kinase | Cell Signaling Technology | Cat# 9202 RRID: AB_331676 |
| Rabbit monoclonal anti-Phospho-EGF Receptor (Tyr1068) (D7A5) | Cell Signaling Technology | Cat# 3777; RRID: AB_2096270 |
| Rabbit polyclonal anti-EGF Receptor | Cell Signaling Technology | Cat# 2232; RRID: AB_331707 |
| Mouse monoclonal anti-b-Actin (8H10D10) | Cell Signaling Technology | Cat# 3700; RRID: AB_2242334 |
| Rabbit polyclonal anti-KIF15 | Thermo Fisher Scientific | Cat# 55407-1-AP; RRID: AB_11182836 |
| Rabbit monoclonal anti-α-Tubulin (11H10) | Cell Signaling Technology | Cat# 2125; RRID: AB_2619646 |
| Rabbit monoclonal anti-COX IV (3E11) | Cell Signaling Technology | Cat# 4850; RRID: AB_2085424 |
| Rabbit anti-Caspase-3 | Cell Signaling Technology | Cat# 9662; RRID: AB_331439 |
| Mouse monoclonal anti-DDDDK-Tag | MBL International | Cat#M185-3L; RRID: AB_11123930 |
| Rabbit anti-Phospho-Histone H3 (Ser 10) | Cell Signaling Technology | Cat# 9701; RRID: AB_331535 |
| Rabbit monoclonal anti-Histone H3 (D1H2) | Cell Signaling Technology | Cat#4499; RRID: AB_10544537 |
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| Bacterial and virus strains | ||
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| PDGF-IRES-Cre retrovirus | Lei et al. (2011); Kenchappa et al. (2020) | NA |
| pAdCMV/v5-DEST-S727D-STAT3-3xFlag plasmid | Addgene | Cat# 99263 |
| pAdCMV/v5-DEST-S727A-STAT3-3xFlag plasmid | Addgene | Cat# 99262 |
| STAT3-C Flag pRc/CMV | Addgene | Cat# 8722 |
| pLenti Lifeact-EGFP BlastR plasmid | Addgene | Cat# 84383 |
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| Chemicals, peptides, and recombinant proteins | ||
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| PDGF-AA | Peprotech | Cat# 100-13A |
| hFGF (Human Fibroblast Growth factor ) | R&D systems | Cat# 233-FB-025 |
| hEGF (Human Epidermal Growth Factor) | Sigma-Aldrich | Cat# E9644 |
| Fibronectin | Millipore-Sigma | Cat# FC010 |
| Heparin | STEMCELL Technologies | Cat# 07980 |
| N2 supplement | Gibco life technologies | Cat# 17502-048 |
| NeuroPlex supplement | Gemini | Cat# 400-161 |
| Ispinesib | Axon Medchem | Cat# 2446-25 |
| SH5-07 | Selleck Chemicals | Cat# S7923 |
| Saracatinib | Selleck Chemicals | Cat# S1006 |
| Dasatinib | Selleck Chemicals | Cat# S1021 |
| Etoposide | Selleck Chemicals | Cat# S1225 |
| Elacridar | Selleck Chemicals | Cat# S7772 |
| Rhodamine Phalloidin | Cytoskeleton | Cat# PHDR1 |
| VECTASHIELD with DAPI | Vector Laboratories | Cat# U-1500 |
| Formaldehyde, 10% methanol free | CHEM (VWR) | Cat# 87001-890 |
| SUPER signal West Pico PLUS Chemiluminescent substrate | Thermo Fisher Scientific | Cat# 34580 |
| PBS | Thermo Fisher Scientific | Cat# 21-040 |
| PVDF membrane | Biorad | Cat# 1620174 |
| DMSO | Corning | Cat# 25-950-cqc |
| BSA | Thermo Fisher Scientific | Cat# 23209 |
| Non-fat dry milk (Blotting grade blocker) | Biorad | Cat# 170-6404 |
| Accutase | Sigma-Aldrich | Cat# A6964-500 |
| Ethanol | Thermo Fisher Scientific | Cat# 61500-0020 |
| Tween 20 | Sigma-Aldrich | Cat# P1379 |
| TBS | Thermo Fisher Scientific | Cat# 28358 |
| Protease Inhibitor Cocktail, EDTA-free (100X) | Thermo Fisher Scientific | Cat# 87785 |
| Pierce T-1step transfer buffer | Thermo Fisher Scientific | Cat# 84731 |
| Western blot striping buffer | Thermo Fisher Scientific | Cat# 46430 |
| 10X Tris/Glycine/SDS buffer | Biorad | Cat# 1610772 |
| Geltrex | Gibco | Cat# A14132-01 |
| Laminin | Thermo Fisher Scientific | Cat# 3400-010-02 |
| RIPA lysis buffer | Thermo Fisher Scientific | Cat# 89900 |
| B27 supplement | Thermo Fisher Scientific | Cat# A3582801 |
| Laemmli SDS Sample Buffer, reducing, 6X | Thermo Fisher Scientific | Cat# AAJ61337AC |
| Anti-Anti (100x) | Gibco | Cat# 15240-062 |
| D-Luciferin | Perkin Elmer | Cat# 122799 |
| Lipofectamine 3000 transfection agent | Life Technologies | Cat# 11668027 |
| Doxorubicin | Selleck Chemicals | Cat# S1208 |
| Blasticidine-S-Hydrochloride | Sigma-Aldrich | Cat# SBR00022 |
| Kif15-IN-1 | Medchem Express | Cat# HY-15948/CS-2697 |
| Nocodazole | EMD Millipore | Cat# 487928 |
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| Critical commercial assays | ||
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| CellTiter-Glo 2.0 assay kit | Promega | Cat# G9242 |
| Pierce™ BCA Protein Assay Kit | Thermo Fisher Scientific | Cat# 23225 |
| RNAqueous™ Total RNA Isolation Kit | Thermo Fisher Scientific | Cat# AM1912 |
| TURBO DNA-free™ Kit | Thermo Fisher Scientific | Cat# AM1907 |
| Mitochondria Isolation Kit for cultured cells | Abcam | Cat# ab110170 |
| Nuclear Extraction Kit | Abcam | Cat# ab113474 |
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| Experimental models: Cell lines | ||
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| Trp53−/− | This paper | NA |
| Trp53/Pten−/− | This paper | NA |
| GBM1A | (Galli et al., 2004) | NA |
| GBML1 | (Deleyrolle et al., 2011) | NA |
| GBM612 | Kenchappa et al. (2020) | NA |
| GBM108 | Gift from Dr. Quiñones-Hinojosa | NA |
| GBM120 | Kenchappa et al. (2020) | NA |
| MES1861 | Reilly et al. (2000); Gürsel et al., 2011 | NA |
| MES4622 | Reilly et al. (2000); Gürsel et al., 2011 | NA |
| PN20 | Hambardzumyan et al. (2009) | NA |
| PN24 | Hambardzumyan et al. (2009) | NA |
| PN62 | Hambardzumyan et al. (2009) | NA |
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| Experimental models: Organisms/strains | ||
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| Trp53fl/fl mice | Jackson Laboratory | Stock# 008462 |
| NOD-SCID mice | Jackson Laboratory | Stock# 005557 |
| Wild type FVB mice | Taconic Biosciences Inc | NA |
| FVB mice | Taconic Biosciences Inc | NA |
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| Software and algorithms | ||
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| Gene Set Enrichment Analysis (GSEA), hallmark analysis (version h.all.v7.0) | Broad Institute | http://software.broadinstitute.org/gsea/index.jsp |
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| Deposited data | ||
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| Bulk and single cell RNA-seq data | Gene Expression Omnibus database |
GSE193180 (all) GSE193178 (bulk RNA-seq) GSE193179 (scRNA-seq) |
| Mass spectrometry proteomics data | ProteomeXchange Consortium | PXZD030715https://doi.org/10.6019/PXD030715 |