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. 2023 Mar 16;14:29. doi: 10.1186/s40104-023-00841-4

Fig. 6.

Fig. 6

Verification of the role of FTL in involvement in DON-mediated ferroptosis in IPEC-J2 cells. Relative mRNA (A), a representative image (B) and the relative density (C) of protein bands of FTL in IPEC-J2 cell after FTL siRNA transfection. IPEC-J2 cell viability was analyzed by CCK-8 after treated by FTL siRNA transfection and DON treatment (D). A representative image (E) and the relative density (F) of protein bands of FTL in IPEC-J2 cell after FTL siRNA transfection and DON treatment. Values are expressed as means ± SD, n = 3–6. Labeled means without a common letter differ, P < 0.05. NC, cells treated with negative control siRNA; siRNA, cells treated with FTL siRNA; NC+DON, cell treated with negative control siRNA plus DON; siRNA+DON, cell treated with FTL siRNA plus DON