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. 2023 Mar 15;17(1):19. doi: 10.1186/s13065-023-00929-y

Table 1.

Effect of experimental parameters on the number of theoretical plates

Parameter Number of theoretical plates (N) Resolution, RS Selectivity, α
ASP ATR RAM ASP/ATR ATR/RAM ASP/ATR ATR/RAM
% Concentration n- propanol (v/v)
6 770 1271 746 2.9 4.2 3.5 2.78
8 510 931 1005 2.7 4.7 3.7 2.9
10 504 971 1317 2.7 4.6 3.1 2.7
12 465 892 1336 1.9 4.42 3.6 3.4
14 423 743 1402 3.5 3.6 16 2.7
15 567 1112 363 5.1 2.7 18 2.2
Concentration of SDS, M
0.05 467 1045 395 4.6 8 3 3.2
0.08 970 1766 1172 4.36 7.2 3.4 3.2
0.1 659 1229 1491 3 5 3.3 3.3
0.12 515 869 1485 2 4.44 4 3.5
0.15 480 859 1158 1.7 3.6 3.75 3.3
0.18 414 658 1143 1.5 2.2 6 3.5
pH of the medium
2.5 515 971 1485 3.2 5 3 2.66
3 651 606 1173 2.2 5.87 3.75 4.1
4 308 1207 207 0.32 6.35 1.17 26.76
5 Overlapped 343
6 Overlapped peaks
Flow rate, mL/min.
0.8 417 1094 forked 2.6 3.36 4.42 2.64
1 469 987 1380 2.38 4.4 4.16 3.12
1.2 458 974 1376 2.8 4.16 4.24 3.76
1.5 515 871 1485 2.54 4.2 4 3.37
2 473 964 1210 2.26 4.34 5.5 3.36