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Parameters of system suitability for the proposed HPLC method for the determination of VAL and AML
| Parameter | ASP/ATR | ATR/IS | IS/RAM | Reference value |
|---|---|---|---|---|
| Retention time, min | 1.5 | 2.3 | 4.3 | |
| Capacity factor, K’ | 0.36 | 1.09 | 2.9 | 0–10 |
| Symmetry factor | 0.718 | 0.829 | 1.05 | ~ 1 |
| Resolution, RS | 2.67 | 2.4 | 2. 2 | RS > 2 |
| Selectivity, α | 3.0 | 1.75 | 1.52 | α > 1 |
| Theoretical plates, N | 400 | 690.93 | 603.75 |
