A) Distribution of the Woolly Sculpin, Clinocottus analis. Woolly Sculpin are found in the rocky shore intertidal from Fort Bragg, California, USA to Punta Abreojos, Baja California, Mexico. The collection site of the sequenced individual, Pacific Grove, California, is indicated by the red star on the map. B) Top: an image of a Woolly Sculpin (photo by P.R. Blaimont). Bottom: image of Franklin Point, California at low tide, an example of representative intertidal habitat for the Woolly Sculpin. C) Distribution of scaffolds of the genome assembly for the Woolly Sculpin. Only the 30 largest scaffolds are shown in decreasing order of size from left to right. Scaffold size is presented in Mega base pairs (Mb). Scaffold 25, which is discussed in the paper, is highlighted in red. D) Bayesian reconstruction of the nonmonophyletic clade that includes the genus Clinocottus using 2 mitochondrial genes (Cyt b, NADH1) and 1 nuclear gene (S7) redrawn from Ramon and Knope (2008). Bootstrap values are shown for Bayesian inference and maximum likelihood, respectively. Clinocottus analis is highlighted in red.