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. 2022 Aug 23;103(8):001771. doi: 10.1099/jgv.0.001771

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© 2022 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.

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Fig. 4. — Antigenic form of WT and stabilized ECs and virions. (a) Gradient-purified WT and recombinant-stabilized viral samples were assessed for the presence of VP0/VP2 by Western blot using mAb 979, n=3 (representative blots shown). (b, c) Fractions were assessed by sandwich ELISA for (b) expanded particles using mAb 979, (c) and native particles using a 16-2-2D scFv. For clarity, fractions are aligned across all panels with wild-type samples on the left and recombinant stabilized samples on the right. For each, n=3 in duplicate, graphed mean±sem, dotted line indicates the background of the assay, wells containing no antigen.