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. 2022 Aug 23;103(8):001771. doi: 10.1099/jgv.0.001771

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© 2022 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.

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Fig. 5. — Thermal stability of WT and stabilized ECs and virions. Samples of virus and EC were heated to a range of temperatures to induce antigenic conversion. Graphed HAg-reactive (a) EC and (b) virus, and NAg-reactive (c) EC and (d) virus. HAg assays use mAb 979 and NAg assays use the 16-2-2D scFv in the detection phase of a sandwich ELISA. Graphed mean±sem normalized to 2× blank well OD, n=3 each in duplicate. WT, wild-type; rs, recombinant stabilized.